TANNING · RECONSTITUTION

Melanotan II dosage calculator

Melanotan II (MT2) dosage calculator with reconstitution math for the standard 10mg lyophilized vial. Loading-phase and maintenance protocol math, live U-100 insulin syringe draw for subcutaneous tanning protocols.

Using the calculator

How to use this Melanotan II dosage calculator

This Melanotan II (MT2) dosage calculator runs reconstitution math live for the standard MT2 vial size — 10mg lyophilized vial — and returns the exact mark to draw to on a 1mL U-100 insulin syringe. The defaults above (10mg vial, 2mL bacteriostatic water, 500mcg target dose) match a typical maintenance-phase MT2 protocol and pull to the 10-unit mark on a U-100 syringe.

Pick your MT2 vial size

Melanotan II is overwhelmingly sold in 10mg lyophilized vials — the standard supplier format. Some 5mg vials exist but are uncommon. Tap the supplier preset to load the standard 10mg vial; override the vial milligrams field if your supplier uses a different size.

Pick your BAC water volume

MT2 reconstitutes cleanly at 2mL of bacteriostatic water for a 10mg vial — a 5 mg/mL concentration where loading-phase doses (250–500 mcg) pull to small, precise marks (5–10U) and maintenance doses (500–1000 mcg) pull to 10–20U. Some users prefer 1mL of BAC (10 mg/mL) for very small loading doses, but the resulting draws (2.5U for a 250mcg dose) are hard to measure precisely on a standard U-100 syringe.

Set your MT2 target dose

Melanotan II uses a two-phase dosing protocol unique to tanning peptides: a loading phase (small daily doses to gradually build pigmentation) followed by a maintenance phase (larger weekly or twice-weekly doses to maintain tan). Loading-phase doses start at 250 mcg daily and titrate up to 500 mcg daily over 2–3 weeks. Maintenance is typically 500–1000 mcg once or twice weekly. Enter your dose in micrograms and the calculator returns U-100 units.

Read the U-100 syringe units

Pull the plunger to the indicated unit mark on a 1mL U-100 insulin syringe. At default settings (10mg/2mL/500mcg dose), the draw is 10U. The visual syringe shows the fill in real time — drag it directly to scrub through the loading-to-maintenance dose range live.

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Complete reference · Melanotan II

Melanotan II (MT2) dosage and reconstitution guide

Melanotan II (MT2) is a synthetic 7-amino-acid analog of α-melanocyte-stimulating hormone (α-MSH) that binds melanocortin receptors to stimulate melanin production in skin and induce tanning. Originally developed at the University of Arizona in the 1990s as a potential pharmacological alternative to UV tanning, MT2 was never brought to market as a tanning agent (its derivative Bremelanotide / PT-141 was developed for a different indication — sexual dysfunction). MT2 is widely used as a research peptide in the cosmetic / tanning context. This guide covers MT2 dose math, the loading-then-maintenance protocol unique to tanning peptides, reconstitution at the standard 10mg vial size, half-life, side-effect profile (nausea, increased libido, mole darkening), the differences between subcutaneous injection and nasal spray delivery, and the differences between Melanotan I and Melanotan II.

Melanotan II dosage protocol — loading and maintenance

MT2 uses a two-phase dosing pattern that is not found with most other peptides. The loading phase uses small daily subcutaneous doses to gradually build melanin pigmentation in the skin over 2–4 weeks. Once the desired pigmentation level is reached, the protocol transitions to maintenance dosing at larger but less frequent intervals. Doses below are common values from research and community protocols, not medical recommendations.

PhaseDoseFrequencyDuration / notes
Loading week 1250 mcg1× daily, subQTolerability assessment — start low
Loading weeks 2–3500 mcg1× daily, subQContinue daily until target tan reached
Loading weeks 3–4 (aggressive)500–750 mcg1× daily, subQFor users wanting faster results
Maintenance (light)500 mcg1× weekly, subQMaintain pigmentation post-loading
Maintenance (standard)500–1000 mcg1–2× weekly, subQMost common maintenance pattern
Pre-sun exposure250–500 mcg24 hours before UVSome users dose before sun exposure

Melanotan II reconstitution — 10mg vial

The 10mg lyophilized vial is the universal MT2 supplier format. Reconstitution at 2mL of bacteriostatic water yields a 5 mg/mL concentration where a 250mcg loading dose pulls to 5U on a U-100 syringe and a 500mcg dose pulls to 10U — both readable marks. 1mL of BAC water yields 10 mg/mL for users who want larger total dose capacity per vial, but very small loading-phase doses become hard to measure (a 250mcg dose at 10 mg/mL is just 2.5U).

STEP 01
Bring vial to room temperature
Lyophilized MT2 ships cold. Allow 10–15 minutes at room temperature before reconstitution.
STEP 02
Wipe both stoppers with alcohol
Both the MT2 vial and the bacteriostatic water vial.
STEP 03
Draw 2mL of BAC water
Use a 3mL drawing syringe with 18–21G needle. For a 10mg vial, 2mL is the standard reconstitution volume.
STEP 04
Inject BAC slowly down the vial wall
Tilt the MT2 vial and inject the bacteriostatic water down the inside wall — never directly onto the lyophilized cake.
STEP 05
Swirl gently until clear
Swirl 30–60 seconds. MT2 dissolves quickly to a clear solution. Do not shake.
STEP 06
Refrigerate
Store at 2–8°C. Reconstituted MT2 is stable for approximately 4 weeks refrigerated. Some users freeze in single-dose aliquots for cycles longer than 4 weeks.
STEP 07
Draw with a U-100 syringe
For a 10mg/2mL vial at 250mcg loading dose: draw to 5U. For 500mcg: draw to 10U. Inject subcutaneously in abdomen, flank, or thigh.

Melanotan II — subcutaneous vs nasal spray

Subcutaneous (subQ) injection is the standard MT2 delivery route — abdomen, flank, or thigh, with site rotation. The dose math above assumes subQ delivery. The needle is shallow (5–8mm) and the injection is essentially painless on a U-100 syringe. Nasal spray MT2 is an alternative delivery format compounded by some suppliers as a needle-free option. Bioavailability of nasal MT2 is significantly lower than subQ (~30–40% absorbed vs subQ baseline), so nasal doses must be roughly 2–3× higher to achieve equivalent systemic effect — and the calculator math doesn't apply directly to nasal delivery. Nasal spray is convenient but less precise; subQ remains the standard for users prioritizing predictable dosing and consistent results.

Melanotan II half-life and dosing timing

MT2 has a serum half-life of approximately 33 hours — long for a small peptide. The long half-life is why maintenance dosing transitions to once or twice weekly after the loading phase: each dose maintains active melanocortin-receptor activation for several days. Loading-phase daily dosing is not pharmacologically necessary based on half-life alone — it accelerates the rate of melanin accumulation in skin by keeping receptor activation continuous through the build-up phase. Once target pigmentation is reached, weekly dosing is sufficient to maintain it.

Melanotan II side effects

MT2 has a distinctive side-effect profile due to its broad melanocortin-receptor agonism. Nausea is common during loading-phase dosing, typically peaks 30–90 minutes after injection, and often improves over the first 1–2 weeks of dosing. Eating before injection and dosing before bed both reduce nausea perception. Facial flushing is common in the first hour after dosing. Increased libido and spontaneous erections in males are well-documented effects of melanocortin-4 receptor activation — Bremelanotide (PT-141), a metabolite of MT2, is FDA-approved specifically for sexual dysfunction based on this mechanism. Mole and freckle darkening occurs because MT2 stimulates melanin production globally, including in existing pigmented lesions. New mole formation has been reported anecdotally and is a theoretical concern. Decreased appetite is common (melanocortin agonism affects central appetite regulation). MT2 should not be used by individuals with a history of melanoma or significant atypical mole burden — pre-existing dermatologic risk should be evaluated before starting.

Melanotan I vs Melanotan II

Melanotan I (afamelanotide, brand name Scenesse) and Melanotan II are different molecules with different applications. Melanotan I is a 13-amino-acid α-MSH analog and is FDA-approved for erythropoietic protoporphyria (a rare light-sensitivity condition). It induces tanning but with significantly fewer side effects than MT2 because it has more selective melanocortin-1 receptor (MC1R) activity — less nausea, less libido effect, less appetite change. Melanotan II is a 7-amino-acid α-MSH analog with broader melanocortin-receptor activity (MC1R, MC3R, MC4R, MC5R), producing both stronger tanning effect AND the broader side-effect profile. MT1 is harder to source as a research peptide and is more expensive; MT2 is the practical choice for tanning research despite the side-effect trade-off.

Melanotan II results and timeline

MT2 results follow a predictable timeline tied to skin cell turnover. Week 1: minimal visible change; first signs of darkening at injection sites. Week 2–3: general skin tone deepening becomes visible, particularly in sun-exposed areas. Loading-phase users typically reach a noticeable tan by week 3. Week 4–6: peak tan develops; existing moles and freckles darken visibly. Maintenance phase: weekly dosing maintains the tan; missed doses cause gradual fade as natural skin turnover (~28 days) replaces pigmented cells. UV exposure during loading dramatically accelerates and deepens results — MT2 sensitizes melanocytes to respond more aggressively to UV, so even brief sun exposure produces more pigmentation than it would without MT2. This is also why MT2 users should be careful with UV exposure during loading: the same sensitization that produces faster tan also produces faster burn risk if unprotected exposure is excessive.

Frequently asked questions

What is the standard Melanotan II dose?
MT2 uses a two-phase protocol. Loading phase: 250–500 mcg subcutaneously once daily for 2–4 weeks until desired pigmentation is reached. Maintenance phase: 500–1000 mcg subcutaneously once or twice weekly to maintain tan. Some protocols dose 250–500 mcg the day before planned UV exposure.
How do you calculate a Melanotan II dose?
MT2 dose calculation uses the same reconstitution math as any peptide: concentration (mg/mL) = vial mg ÷ BAC mL; injection volume (mL) = dose mg ÷ concentration; U-100 syringe units = injection volume × 100. For a 10mg vial reconstituted with 2mL of BAC water and a 500mcg target dose: concentration is 5 mg/mL, injection volume is 0.1 mL, draw to 10 units on a U-100 syringe.
How much bacteriostatic water do I add to a 10mg MT2 vial?
2mL of bacteriostatic water is the standard reconstitution volume for a 10mg MT2 vial. This yields a 5 mg/mL concentration where a 250mcg loading dose pulls to 5U and a 500mcg dose pulls to 10U on a U-100 syringe — both readable marks.
How many units is 500mcg of MT2?
It depends on concentration. For a 10mg vial reconstituted with 2mL BAC water (5 mg/mL): 500mcg pulls to 10 units on a U-100 syringe. For 1mL BAC (10 mg/mL): 5 units. The calculator above computes this live.
How long does it take MT2 to work?
Most users see initial darkening within 1–2 weeks of starting daily loading-phase dosing. A noticeable tan typically develops by week 3. Peak pigmentation develops over weeks 4–6. UV exposure during loading dramatically accelerates results.
What is the MT2 half-life?
Melanotan II has a serum half-life of approximately 33 hours — long for a small peptide. The long half-life is why maintenance dosing transitions to once or twice weekly after the loading phase; each dose maintains receptor activation for several days.
What are MT2 side effects?
The most common are nausea during loading-phase dosing (typically improves over 1–2 weeks), facial flushing in the first hour after injection, increased libido and spontaneous erections in males, and darkening of existing moles and freckles. Decreased appetite is common. MT2 should not be used by individuals with a history of melanoma or significant atypical mole burden.
Can MT2 be used as a nasal spray?
Yes. Some suppliers compound MT2 as a nasal spray as a needle-free alternative. Bioavailability is significantly lower than subcutaneous injection (~30–40%), so nasal doses must be 2–3× higher to achieve equivalent systemic effect. Nasal spray is convenient but less precise than subQ; subQ remains the standard for users prioritizing predictable dosing.
What is the difference between Melanotan I and Melanotan II?
Melanotan I (afamelanotide) is a 13-amino-acid α-MSH analog with more selective melanocortin-1 receptor activity — produces tanning with fewer side effects. It is FDA-approved for erythropoietic protoporphyria. Melanotan II is a 7-amino-acid α-MSH analog with broader melanocortin-receptor activity (MC1R + MC3R + MC4R + MC5R), producing stronger tanning AND a broader side-effect profile (nausea, libido, appetite). MT2 is the practical choice for tanning research; MT1 is harder to source and more expensive.
When should you inject MT2?
Most users dose MT2 before bed during the loading phase to allow nausea (if any) to occur during sleep. Eating before injection significantly reduces nausea perception. During maintenance phase, timing is less critical due to MT2's long half-life — any consistent weekly schedule works.
Does MT2 cause new moles?
MT2 stimulates melanin production globally, which causes existing moles and freckles to darken visibly. New mole formation has been reported anecdotally during MT2 use, though formal research literature on this risk is limited. Individuals with significant atypical mole burden, family history of melanoma, or personal history of skin cancer should not use MT2 without dermatologic evaluation. Users on MT2 should monitor existing moles for changes (the standard ABCDE criteria) and have a baseline full-body skin exam before starting.
How long should you cycle MT2?
MT2 doesn't have a defined "cycle" structure like research peptides such as BPC-157. Users typically run a loading phase to reach desired pigmentation (2–4 weeks), then maintain weekly dosing as long as they want to maintain the tan. Stopping completely allows natural skin turnover (~28 days) to gradually fade pigmentation back to baseline over 1–3 months. Some users do seasonal cycles (load in spring, maintain through summer, stop in fall).
RESEARCH USE ONLY. This calculator and the information on this page are provided for informational and research purposes only. Consult a licensed medical provider before administering any peptide. PeptideMaxxers does not manufacture, sell, or ship peptides. Doses, cycle lengths, and protocols referenced above are common values from published research and community sources — they are not medical recommendations.