GLP-1 / GIP / GLUCAGON · RECONSTITUTION

Retatrutide dosage calculator

Retatrutide (LY3437943) dosage calculator with reconstitution math for 5mg and 10mg lyophilized vials. Eli Lilly triple agonist in Phase 3 trials. Live U-100 insulin syringe draw with Phase 2 titration schedule.

Using the calculator

How to use this Retatrutide dosage calculator

This Retatrutide dosage calculator runs reconstitution math live for the most common research Retatrutide vial sizes — 5mg and 10mg lyophilized vials — and returns the exact mark to draw to on a 1mL U-100 insulin syringe. The defaults above (5mg vial, 2mL bacteriostatic water, 0.5mg target dose) match common Phase 2 study starting protocols and pull to the 20-unit mark on a U-100 syringe.

Pick your Retatrutide vial size

The supplier presets cover the two vial sizes commonly available in research formats: 5mg lyophilized vial (the standard) and 10mg lyophilized vial (for extended titration). Phase 2 clinical trials titrated from 0.5 mg up to 12 mg weekly across 36 weeks — the 10mg vial is necessary to reach the higher trial doses without weekly vial changes.

Pick your BAC water volume

Retatrutide reconstitutes well at 2mL of bacteriostatic water for a 5mg vial — a 2.5 mg/mL concentration where 0.5mg pulls to 20U, 2mg pulls to 80U on a U-100 syringe. The 10mg vial reconstituted with 2mL yields 5 mg/mL (halves every draw — 0.5mg = 10U, 2mg = 40U). Lower BAC water volumes produce hard-to-read draws at the very low starting doses.

Set your Retatrutide target dose

Retatrutide is dosed in milligrams — switch the unit toggle to mg. Phase 2 trials used a strict titration from 0.5 mg weekly up through 2, 4, 8, and 12 mg weekly to manage gastrointestinal tolerability. Most research protocols follow this titration schedule. Enter your current dose and the calculator returns U-100 units, injection volume, concentration, and doses per vial.

Read the U-100 syringe units

Pull the plunger to the indicated unit mark on a 1mL U-100 insulin syringe. At default settings (5mg/2mL/0.5mg dose), the draw is 20U. The visual syringe shows the fill in real time — drag it directly to scrub through doses live during titration planning.

PEPTIDEMAXXERS · IOS · IPADOS

The full protocol in your pocket.

Reconstitution math is step one. Running a peptide protocol means reminders, rotation, adherence, and history. PeptideMaxxers handles all of it — offline-first, privacy-first, native SwiftUI.

Download on the
App Store
iOS 17+ · 12 MB · ★ 4.9
Dose logging
One-tap injection logs with site, time, note.
Cycle scheduling
Titrations, alternating cadences, split dosing.
Push reminders
Exact draw (e.g. 25U on U-100) right in the banner.
HealthKit sync
Mirror doses into Apple Health automatically.
Adherence streaks
12-week streak grid — see your consistency.
iCloud backup
Protocols and logs sync across iPhone + iPad.
PeptideMaxxers iOS app — onboarding screen with reconstitution math preview
PeptideMaxxers iOS app — peptide library showing 23 compounds with categories and half-life
PeptideMaxxers iOS app — Tirzepatide dose logged confirmation
WEB CALCULATOR · VS · PEPTIDEMAXXERS APP

This calculator handles the math. The app handles the protocol.

FEATURE
WEB · FREE
APP · $
Real-time reconstitution math
23-compound preset library
Visual U-100 syringe
Dose logging + injection history
Injection-site rotation tracking
Cycle / protocol scheduler
Push reminders before every dose
HealthKit sync
Offline + iCloud backup
Adherence analytics + streaks
STILL ON THE FENCE?
Run your full Retatrutide protocol in the app — dose logs, site rotation, reminders, cycle tracking.
Download on the
App Store
Complete reference · Retatrutide

Retatrutide dosage and reconstitution guide

Retatrutide (LY3437943) is Eli Lilly's investigational triple-agonist peptide that simultaneously activates the GLP-1, GIP, and glucagon receptors — the first triple agonist to reach Phase 3 clinical trials. Phase 2 results published in 2023 demonstrated 24% mean body weight reduction at 48 weeks in the 12 mg arm, the largest weight-loss effect ever reported in a clinical trial of an investigational obesity therapy. Retatrutide is currently in Phase 3 trials with FDA approval expected in 2026 or 2027. This guide covers Retatrutide dose math, the Phase 2 titration schedule, reconstitution at 5mg and 10mg vial sizes, side effects from clinical trial data, the Retatrutide vs Tirzepatide comparison (the dual agonist Lilly already markets as Mounjaro/Zepbound), cost and availability, and the FDA approval timeline.

Retatrutide dosing protocol — Phase 2 titration schedule

Phase 2 clinical trials titrated Retatrutide from 0.5 mg weekly up through escalating doses to manage gastrointestinal tolerability — primarily nausea, which is the dose-limiting side effect of all GLP-1 / GIP / glucagon agonists. The titration schedule below mirrors the Phase 2 trial protocol and is the standard reference for research dosing. Doses are not medical recommendations.

Phase / weekWeekly doseFrequency / routeNotes
Initiation (weeks 1–4)0.5 mg1× weekly, subQTolerability assessment
Titration step 1 (weeks 5–8)2 mg1× weekly, subQMost users tolerate
Titration step 2 (weeks 9–12)4 mg1× weekly, subQSome GI side effects
Maintenance (low)4–8 mg1× weekly, subQStandard maintenance range
Maintenance (mid)8 mg1× weekly, subQPhase 2 mid-arm dose
Maintenance (high)12 mg1× weekly, subQPhase 2 high-arm dose — 24% weight loss at 48 weeks

Retatrutide reconstitution — 5mg and 10mg vials

The 5mg lyophilized vial is the standard research Retatrutide format. Reconstitution at 2mL of bacteriostatic water yields a 2.5 mg/mL concentration; a 0.5mg starting dose pulls to 20U on a U-100 syringe. The 10mg vial reconstituted with 2mL yields 5 mg/mL (0.5mg = 10U, 4mg = 80U), which is the practical choice for users running titration schedules that reach the 8–12 mg arms — a 5mg vial doesn't hold a single 12mg dose, so 10mg vials become necessary at higher doses.

STEP 01
Bring vial to room temperature
Lyophilized Retatrutide ships cold. Allow 10–15 minutes at room temperature before reconstitution.
STEP 02
Wipe both stoppers with alcohol
Both the Retatrutide vial and the bacteriostatic water vial.
STEP 03
Draw BAC water
2mL for a 5mg or 10mg vial. Use a 3mL drawing syringe with 18–21G needle.
STEP 04
Inject BAC slowly down the vial wall
Tilt the Retatrutide vial and inject the bacteriostatic water down the inside wall — never directly onto the lyophilized cake.
STEP 05
Swirl gently until clear
Swirl 30–60 seconds. Retatrutide dissolves to a clear solution. Do not shake — Retatrutide is a 39-amino-acid modified peptide and aggressive agitation can affect potency.
STEP 06
Refrigerate
Store at 2–8°C. Reconstituted Retatrutide is stable for approximately 4 weeks refrigerated; aliquoting and freezing extends shelf life for users running long protocols at low weekly doses.
STEP 07
Draw weekly dose with U-100 syringe
Retatrutide is dosed once weekly. For a 5mg/2mL vial at 0.5mg starting dose: draw to 20U. Inject subcutaneously in abdomen, thigh, or upper arm.

Retatrutide vs Tirzepatide

Retatrutide and Tirzepatide are both Eli Lilly compounds in the same incretin-mimetic family, but they differ in mechanism. Tirzepatide (marketed as Mounjaro for diabetes and Zepbound for obesity) is a dual agonist activating GLP-1 and GIP receptors. Retatrutide is a triple agonist activating GLP-1, GIP, AND glucagon receptors. The added glucagon agonism is the differentiator — glucagon receptor activation increases energy expenditure and lipolysis, which is theorized to drive Retatrutide's larger weight-loss effect. Phase 2 head-to-head data: Tirzepatide produced ~21% body weight reduction at 72 weeks (15 mg arm); Retatrutide produced 24% body weight reduction at 48 weeks (12 mg arm) — a faster and larger effect, though longer-term Phase 3 data is pending. Both compounds use the same weekly subQ injection protocol with similar titration logic and similar GI side-effect profiles. Retatrutide is not yet FDA-approved; Tirzepatide is approved and commercially available.

Retatrutide side effects

Phase 2 trial data identified the same gastrointestinal side-effect cluster seen with all GLP-1 / GIP agonists, with intensity scaling with dose and titration speed. Nausea was the most common adverse event, peaking during titration steps and partially adapting during maintenance. Vomiting and diarrhea occurred in a minority and were more frequent at the 8–12 mg arms. Constipation was reported by some users on lower doses. Decreased appetite is the intended therapeutic effect and is universal. Mild heart rate elevation (~3–5 bpm) was observed. Liver enzyme elevations were observed in a small fraction of trial participants and are being monitored in Phase 3. Slow titration significantly mitigates GI tolerability issues — the Phase 2 protocol's 4-week dose-step intervals are the standard for managing these effects.

Retatrutide cost and availability

Retatrutide is not currently FDA-approved and is not commercially available through standard pharmacy channels. Research-format Retatrutide (lyophilized vials from research-chemical suppliers) is the only access pathway pre-approval and exists in a regulatory grey area — distinct from the established research-peptide market for compounds like BPC-157 or TB-500 because Retatrutide is an active Phase 3 pharmaceutical with an identified manufacturer (Eli Lilly). Pricing varies widely by supplier and vial size, typically $80–$200 per 5mg vial as of 2026. Once FDA-approved, expect the commercial product to follow Tirzepatide's pricing model (~$1,000–$1,200/month list price; insurance coverage variable) and the research-format market to contract significantly as it did for semaglutide post-approval.

Retatrutide FDA approval timeline

Retatrutide entered Phase 3 trials in mid-2023, with the primary obesity trials (TRIUMPH-1, TRIUMPH-2, TRIUMPH-3, TRIUMPH-4) covering different patient populations and durations. Eli Lilly has guided that primary endpoint data is expected through 2026, with FDA submission likely in late 2026 and approval possible in 2027 — though regulatory timelines for high-profile metabolic drugs can move faster. Approval is expected first for obesity, with diabetes and additional cardiometabolic indications potentially following. The compound's Phase 2 data (24% weight loss at 48 weeks) significantly exceeded expectations and has driven elevated investor and clinical attention.

Retatrutide mechanism of action

Retatrutide's triple-agonist mechanism activates three distinct receptors involved in glucose, appetite, and energy regulation. GLP-1 receptor activation suppresses glucagon, slows gastric emptying, and reduces appetite via central pathways — the same mechanism semaglutide uses. GIP receptor activation enhances insulin secretion in response to glucose and is thought to improve adipose tissue insulin sensitivity — the addition that distinguishes Tirzepatide from earlier GLP-1 monoagonists. Glucagon receptor activation increases hepatic glucose output (counter-intuitive in a diabetes drug) but also significantly increases energy expenditure and lipolysis — the addition that distinguishes Retatrutide from Tirzepatide and is theorized to drive the larger weight-loss effect. The risk of adding glucagon agonism is the potential for hyperglycemia in diabetic patients; Phase 2 data showed Retatrutide actually improved glycemic control because the GLP-1 component dominates, but this is a key safety question for Phase 3.

Frequently asked questions

What is the standard Retatrutide dose?
Phase 2 clinical trials used weekly doses ranging from 0.5 mg (initiation) up to 12 mg (highest tested arm). Most research protocols follow the trial titration: 0.5 mg weekly for 4 weeks, then 2 mg, then 4 mg, then 8 mg or 12 mg as tolerated. The 12 mg weekly arm produced 24% body weight reduction at 48 weeks in Phase 2.
How do you calculate a Retatrutide dose?
Retatrutide dose calculation uses the same reconstitution math as any peptide: concentration (mg/mL) = vial mg ÷ BAC mL; injection volume (mL) = dose mg ÷ concentration; U-100 syringe units = injection volume × 100. For a 5mg vial reconstituted with 2mL of BAC water and a 0.5mg target dose: concentration is 2.5 mg/mL, injection volume is 0.2 mL, draw to 20 units on a U-100 syringe.
How much bacteriostatic water do I add to a 5mg Retatrutide vial?
2mL of bacteriostatic water is the most common reconstitution volume for a 5mg Retatrutide vial. This yields a 2.5 mg/mL concentration where doses pull to readable U-100 marks across the typical Phase 2 dose range (0.5mg = 20U, 2mg = 80U). For users running protocols that reach 8–12 mg weekly, the 10mg vial reconstituted with 2mL (5 mg/mL) is more practical.
How many units is 0.5mg of Retatrutide?
It depends on concentration. For a 5mg vial reconstituted with 2mL BAC water (2.5 mg/mL): 0.5mg pulls to 20 units on a U-100 syringe. For a 10mg vial with 2mL BAC (5 mg/mL): 10 units. The calculator above computes this live for any input.
What is the difference between Retatrutide and Tirzepatide?
Tirzepatide is a dual agonist (GLP-1 + GIP receptors). Retatrutide is a triple agonist (GLP-1 + GIP + glucagon receptors). The added glucagon agonism in Retatrutide increases energy expenditure and lipolysis, which is theorized to drive its larger Phase 2 weight-loss effect (24% at 48 weeks vs Tirzepatide's 21% at 72 weeks). Both use weekly subQ injection. Tirzepatide is FDA-approved; Retatrutide is in Phase 3.
When will Retatrutide be FDA-approved?
Eli Lilly has guided primary Phase 3 endpoint data through 2026, with FDA submission likely in late 2026 and approval possible in 2027. Approval is expected first for obesity, with potential follow-on indications for diabetes and cardiometabolic disease. Timelines can move faster for high-profile metabolic drugs.
What are Retatrutide side effects?
The primary side effects from Phase 2 trials are gastrointestinal — nausea (most common), vomiting and diarrhea (less frequent, more common at higher doses), constipation, and decreased appetite (intended). Mild heart rate elevation (~3–5 bpm) was observed. Liver enzyme elevations occurred in a small subset and are being monitored in Phase 3. Slow titration significantly mitigates GI tolerability issues.
How does Retatrutide work?
Retatrutide is a triple agonist activating three receptors. GLP-1 receptor activation suppresses appetite and slows gastric emptying. GIP receptor activation enhances glucose-dependent insulin secretion. Glucagon receptor activation increases energy expenditure and lipolysis — the differentiator from Tirzepatide. The combined effect produces the largest weight-loss effect of any investigational obesity therapy in clinical trials.
How long is a Retatrutide cycle?
Phase 2 and Phase 3 trials use continuous weekly dosing for 48–88 weeks. There is no defined "cycle" structure for Retatrutide as there is for some research peptides — the compound is intended for chronic obesity management, like Tirzepatide. Some users running off-label research protocols cycle off after 12–24 weeks, but this is not the clinical use pattern.
How much weight do you lose on Retatrutide?
Phase 2 trial data: 24% mean body weight reduction at 48 weeks in the 12 mg weekly arm. The 8 mg arm produced approximately 22% reduction; the 4 mg arm approximately 17%; the 1 mg arm approximately 9%. These figures are mean values from the trial population; individual results vary substantially with adherence, dose, baseline weight, and time on treatment.
How much does Retatrutide cost?
Research-format Retatrutide pricing varies widely by supplier and vial size, typically $80–$200 per 5mg vial as of 2026. The compound is not yet FDA-approved and is not available through standard pharmacy channels. Once approved, expect commercial pricing to follow Tirzepatide's model (~$1,000–$1,200/month list price; insurance coverage variable).
Is Retatrutide a GLP-1?
Retatrutide includes GLP-1 receptor agonism but is not a pure GLP-1 agonist — it is a triple agonist activating GLP-1, GIP, and glucagon receptors simultaneously. Pure GLP-1 agonists (semaglutide, liraglutide) activate only the GLP-1 receptor; dual agonists (tirzepatide) activate GLP-1 and GIP; Retatrutide is the first triple agonist to reach Phase 3 trials.
RESEARCH USE ONLY. This calculator and the information on this page are provided for informational and research purposes only. Consult a licensed medical provider before administering any peptide. PeptideMaxxers does not manufacture, sell, or ship peptides. Doses, cycle lengths, and protocols referenced above are common values from published research and community sources — they are not medical recommendations.